PhD student (m/f/d) on mechanical and epigenetic stimulation to direct differentiation of iPSCs towards MSCs
The part-time position 65% starts on the 1st of July 2025 and is initially limited for 3 years with an option to extend.
Our profile:
The Institute for Stem Cell Biology explores how epigenetic mechanisms govern cell fate decisions. Through interdisciplinary research, we investigate how biomaterials and mechanical forces direct cellular differentiation (www.stemcellbiology.ukaachen.de). You will be part of the DFG-funded graduate school Mechanobiology in Epithelial 3D Tissue Constructs (MEƎT; https://me3t.rwth-aachen.de/) working on project A1 “Mechanostimulation to direct differentiation of iPSCs and iPSC-derived embryoid bodies”.
PhD project:
Generation of iPSC-derived mesenchymal stromal cells (iMSCs) raises high expectations for tissue engineering and regenerative medicine. However, achieving directed and uniform differentiation towards iMSCs remains a major challenge. In this PhD project, we aim to leverage hydrogels, cell-cell interactions, and epigenetic editing techniques to enhance directed differentiation within three-dimensional culture systems. Building upon our previous research, we will explore various hydrogel compositions to optimize the generation of iMSCs. Furthermore, we plan to analyze the DNA methylation patterns that are characteristic of MSCs and employ epigenetic editing strategies targeting specific CpG sites to support directed differentiation. This project aims to deepen our understanding of epithelial-to-mesenchymal transition processes and enhance the generation of more uniform iMSCs.
Culturing of iPSCs and differentiation toward specific lineages
Handling of hydrogels and biomaterials
CRISPR-based epigenetic editing technologies
Bioinformatic DNA methylation analysis
Cellular characterization with molecular biology methods
Functional analysis of specific epigenetic modifications
Very good master’s degree in either Biology, Biotechnology, Natural sciences or comparable degrees
Experience with iPSC culture is favourable (but not a prerequisite)
Bioinformatic skills and basic knowledge in R is favourable (but not a prerequisite)
Able to communicate clearly in English and willing to work in an international team
Willingness to interact with other research groups
We provide a highly interactive and well-equipped environment, in an international team, with excellent partners, a broad spectrum of methods and a highly relevant research topic
We offer performance-based remuneration in accordance with the provisions of TV-L (EG 13 65%), including attractive public service benefits.
This position is not gender specific. The RWTH Aachen University Hospital promotes equal opportunities and diversity. If suitably qualified, people with a registered disability will receive priority.