Your role
The successful candidate will work on a cutting-edge experimental research project in the structure-function analysis of chromatin in archaea. The goal of the project is to understand how eukaryotes evolved histone tails and tail-based regulatory mechanisms from an archaeal ancestor, and what role could the tails play in proto-eukaryotic chromatin. Practically, we will merge tail-less histones of a model archaeon with selected eukaryotic or Asgard histone tails in vitro and in vivo and investigate the structural and functional effects on a molecular level.
The project involves a combination of biochemistry, biophysics, and structural biology work. Structural part of the project will involve cryo-EM single particle analysis of nucleosomes, as well as FIB-milling of archaeal cells, and cryo electron tomography (cryo-ET) and subtomogram averaging of chromatin fibres.
Moreover, this project is a close collaboration with the laboratory of Naomichi Takemata (Kyoto University), and will involve regular communication and scientific exchange with the collaborators.
Closing date: 31 July 2024
1. Contract duration: 3 years
2. Grading: Year 1 stipend - €4,010 per month after tax
3. Reference number: HD02627
Related
You have
4. PhD in structural biology, molecular biology, biochemistry, or related disciplines (or you are close to finishing your PhD).
5. Experience in cryo-EM/cryo-ET data acquisition and processing.
6. Experience in biochemistry (protein expression & purification, DNA-protein complex characterization).
7. Confidence in working via command line.
8. Strong interest in the research topic, high motivation and commitment.
9. Ability to work independently and creatively, in an international team with a highly collaborative atmosphere.
10. Excellent communication and presentation skills, fluency in English.
You might also have
11. Experience in microbiology and handling of anaerobic microorganisms.
12. Basic programming skills.
13. Familiarity with HPC computing.